Microsatellite DNA markers for analysis of genetic population structure of stellate sturgeon (Acipenser stellatus Pallas, 1771) in the North (Volga and Ural Rivers) and South Caspian Sea (Sefidrud and Gorganrud Rivers)

نویسندگان

  • M. Pourkazemi
چکیده مقاله:

This study represents a large-scale population genetic analysis of the stellate sturgeon, Acipenser stellatus, in the Caspian Sea. In total, 192 samples of adult stellate sturgeon were collected from four commercial catch stations in the Northern (Volga and Ural Rivers) and Southern Caspian Sea (estuary of Sefidrud and Gorganrod Rivers-Iran). Fifteen sets of microsatellite primers developed from lake sturgeon (Acipenser fulvescens) and shovelnose sturgeon (Scaphirhynchus platorynchus) were tested on genomic DNA. Ten primer sets (LS-19, 34, 54, 68, Spl-105, 104, 163, 170, 173, 113) revealing polymorphic loci were used to analyze the genetic variation found in adults of the stellate sturgeon populations. Analyses revealed that the average number of alleles per locus was 13.05 (range 8 to 18 alleles per locus in regions, Ne= 7.86). All sampled regions contained private alleles. The average observed and expected heterozygosities were 0.665 and 0.862, respectively. Deviations from Hardy-Weinberg equilibrium were seen in most cases. Average of Fis, Fit and Nm were 0.230, 0.261 and 7.498, respectively. Pairwise Population FST Values ranged from 0.019 to 0.035. FST, RST, and gene flow estimates in AMOVA indicated significant genetic differentiation among and regions, indicating that the populations were divergent. The genetic distance between populations indicates that the genetic difference among the studied populations is pronounced. These results together with highly significant RST of genotypic differences between these pairs of collections support the existence of different genetic populations along the Caspian Sea coast. 

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Application of microsatellite markers to study the genetic structure of stellate sturgeon populations (Acipenser stellatus Pallas, 1771) in the south Caspian Sea

Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 a...

متن کامل

Application of microsatellite markers to study the genetic structure of stellate sturgeon populations (Acipenser stellatus Pallas, 1771) in the south Caspian Sea

Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 a...

متن کامل

Gastrointestinal microflora of captured stellate sturgeon (Acipenser stellatus, Pallas, 1771) from Southeast Caspian Sea, Iran

Acipenser stellatus is one of the most invaluable species of Sturgeon fishes in the world. The purpose of this study was to identify the resident microbial community from the gastrointestinal tract (GIT) of Acipenser stellatus. One hundred and twenty three fungi were isolated from the GIT of 7 Acipenser stellatus samples including Aspergillus spp (45.5%), Trichoderma spp (8.9%) and Cladophialop...

متن کامل

Gastrointestinal microflora of captured stellate sturgeon (Acipenser stellatus, Pallas, 1771) from Southeast Caspian Sea, Iran

Acipenser stellatus is one of the most invaluable species of Sturgeon fishes in the world. The purpose of this study was to identify the resident microbial community from the gastrointestinal tract (GIT) of Acipenser stellatus. One hundred and twenty three fungi were isolated from the GIT of 7 Acipenser stellatus samples including Aspergillus spp (45.5%), Trichoderma spp (8.9%) and Cladophialop...

متن کامل

Genetic diversity analysis of aquaculture strains of Acipenser stellatus (Pallas, 1771) using DNA markers

Acipenser stellatus (stellate sturgeon) that has been intensively captured for decades represents a sturgeon species of great importance for the scientific community and fishermen. Besides overfishing and poaching, their reproduction sites have been destroyed and their natural habitats were affected by pollution. In consequence, wild populations of stellate sturgeon have decreased dramatically ...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 15  شماره 2

صفحات  687- 700

تاریخ انتشار 2016-04-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023